Volume 25, Issue 4 (3-2013)                   3 2013, 25(4): 484-492 | Back to browse issues page

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Abstract:   (2993 Views)
In this study the inhibitory effect of metabolites produced by<em> </em><em>Lactobacillus rhamnosus</em> GG (probiotic bacteria) on the growth of human colon cancer cell line CacoII was examined. After accuracy examination of bacterial genus and species MRS(DE Man ,Rogosa &amp; Sharpe) broth culture from<em> </em><em>L. rhamnosus</em> GG was prepared and two supernatant from MRS broth culture which was incubated for 24,48 and 72 hours were provided.One of these supernatant with normal pH(pH=4) and the other one with using NaoH 1N was reached to pH 7.CacoII cells were seeded on 96-well microplates at 8&amp;times;10<sup>3 </sup>cells/well and obtained supernatants at concentrations of 100,200 and 300 &amp;micro;l/mL were inoculated to CacoII cell culture. In average ,the metabolites of <em>L. rhamnosus</em> GG at concentrations of 100, 200 and 300 &amp;micro;l/mL inhibited the growth of CacoII cells by 60.8%,71.5% and 82.1% respectively.
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Type of Study: Research |
Received: 2016/12/25 | Published: 2013/03/15